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To test the efficiency methods implemented in NEXT-RNAi (details are available here) we used a set of 2,431 knockdown-validated siRNAs (from Huesken et al.) and compared NEXT-RNAi's efficiency predictions with inhibitory activities measured. Both, the rational and weighted methods showed a good correlation (Pearson correlation of 0.52 and 0.51, respectively) with the measured inhibitory activity of the siRNAs.

The complete NEXT-RNAi reports are available here: