E-RNAi
Child pages
  • Evaluation of RNAi reagents

Versions Compared

Old Version 10

changes.mady.by.user Thomas Horn

Saved on

compared with

New Version Current

changes.mady.by.user Thomas Horn

Saved on

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.
Comment: Migrated to Confluence 5.3


This E-RNAi option allows to evaluate/re-map existing long dsRNA- or siRNA sequences.


Page contents

Table of Contents


Selection of reagent and organism



Section
Column
width275px

Select type of RNAi reagent

Column
width525px

Choose long dsRNAs for the evaluation of long dsRNAs (including esiRNAs) or siRNAs for the evaluation of short interfering RNAs.

Section
Column
width275px

Select organism

Column
width525px

Select the organism used for the RNAi experiment.


Paste or upload a reagent sequence

It is recommended to paste reagent sequences in FASTA format. Raw sequences can also be pasted, however a query sequence must not contain any newline (each newline is interpreted as new query by E-RNAi).
Alternatively, reagent sequences can also be uploaded from file. Files must contain sequences in FASTA format and *.fa, *.fasta and *.txt file extensions are allowed only.
In the example below three reagent sequences from the HFA/DRSCv1 library were pasted in FASTA format.



After submitting the query, E-RNAi shows the queried reagent sequences as genome browser visualization (if they could be mapped).




The 'Submit Selection' button links to the settings page.